virotype® IHNV/VHSV RT-PCR Kit

For simultaneous detection and differentiation of RNA from Infectious Hematopoietic Necrosis Virus (IHNV) and Viral Hemorrhagic Septicemia Virus (VHSV) / (Availability/distribution: Outside the U.S. and Canada)

Infectious Hematopoietic Necrosis Virus (IHNV) and Viral Hemorrhagic Septicemia Virus (VHSV) are two of the most serious pathogens affecting salmonid populations with high mortality and effects on production leading to serious economic impacts in the fish industry. Frequently tested together, INDICAL’s virotype IHNV/VHSV RT-PCR Kit enables simultaneous detection of RNA from IHNV and VHSV, reducing the number of tests and hands-on time while speeding up time to result and providing confidence in results compared to lab developed tests.

Features and specifications

• Triplex real-time RT-PCR Kit for rapid and highly reliable detection of all known IHNV and VHSV genotypes
• Fast RT-PCR run time ~68 mins
• Endogenous Internal Control specific for salmonids (β-actin) gives confidence in result interpretation
• High sensitivity and specificity
• Ready-to-use PCR mix in an easy-to-use format, no additional pipetting required
• Positive and Negative Controls included
• First and only multiplex IHNV/VHSV RT-PCR Kit approved in Germany
  by the Friedrich-Loeffler-Institut (FLI-C 133)

Infection of fish with IHNV and VHSV is notifiable to the World Organization of Animal Health (WOAH), and various countries and trading areas, like the European Union, have particular legislation in place for the control of the disease (WOAH, 2017c; EU, 2006/88; EU, 2009/177). Furthermore, a differentiation of the two diseases based on clinical and pathological/anatomical symptoms is not possible. Therefore, monitoring and surveillance to detect and identify IHNV and VHSV are legally defined to protect fish cultures.

• Test Principle :  Real-Time PCR
• Packaging :  100
• Pathogenic :  Viral Hemorrhagic Septicemia Virus