INgene q Paratuberculosis (multiplex)

INgene® q Paratuberculosis (multiplex) is a real-time PCR kit for the detection of PTBC F57 and PTBC IS900 DNA in biological samples from Cattle, Small ruminants. The assay is optimized for samples like faeces, rectal swabs, milk, tissues including intestine, ileocecal valve, lymph nodes and strains/vaccines. The kit consists on a multiplex real-time PCR in one-well format, detecting F57 gene in FAM channel and IS9 gene in Cy5 channel. In addition, the mix includes and Endogenous control (EC) that is amplified in HEX/VIC, allowing identification of false negatives due to poor extraction and/or PCR inhibition.

Main assay benefits are:

• Easy to use format, minimun preparation time
• Harmonized thermal profile across most INgene® q kits
• Broadly compatible with available PCR instruments
• Optional quantification with the positive control
• Validated with clinical samples
• Extended shelf life: 18 months
• 100 rxn format